primary antibody p-erk Search Results


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Special primer sequences of target genes used in the present study for qRT-PCR.
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Special primer sequences of target genes used in the present study for qRT-PCR.
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Special primer sequences of target genes used in the present study for qRT-PCR.
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Special primer sequences of target genes used in the present study for qRT-PCR.
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Affinity Biosciences primary antibodies against β-actin, gnai2, pka, erk, p-erk, creb, p-creb
Special primer sequences of target genes used in the present study for qRT-PCR.
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Special primer sequences of target genes used in the present study for qRT-PCR.
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Image Search Results


Special primer sequences of target genes used in the present study for qRT-PCR.

Journal: Poultry Science

Article Title: Dietary resveratrol supplementation alleviates cold exposure-induced pyroptosis and inflammation in broiler heart by modulating oxidative stress and endoplasmic reticulum stress

doi: 10.1016/j.psj.2024.104203

Figure Lengend Snippet: Special primer sequences of target genes used in the present study for qRT-PCR.

Article Snippet: The membranes with proteins were blocked using a 5% of skim milk for 2 h, and then incubated with the corresponding primary antibodies against PERK (WL03378, 1:1000, Wanleibio, China), ATF4 (WL02330, 1:1000, Wanleibio, China), CHOP (WL00880, 1:1000, Wanleibio, China), NLRP3 (WL02635, 1:1500, Wanleibio, China), Caspase1 (WL02996, 1:1000, Wanleibio, China), GSDMD (AF4012, 1:1000, Affinity, China), IL-18 (WL01127, 1:1500, Wanleibio, China), IL-1β (WL00891, 1:500, Wanleibio, China), IL-10 (WL03088, 1:1000, Wanleibio, China), TNF-α (WL01581, 1:1500, Wanleibio, China), and β-actin (ab8227, 1:7000, Abcam, UK) for 12 h at 4°C.

Techniques: Sequencing

mRNA and protein expression levels of genes related to endoplasmic reticulum stress in broiler heart from each group. (A) Relative mRNA expression of GRP78, IRE1, PERK, EIF-2α, ATF4, ATF6, and CHOP in heart tissues. (B) Protein bands and expression levels of PERK, ATF4, and CHOP in heart tissues. a,b,c,d,e Bars with different superscripts in the same index are significantly different at P < 0.05, and bars with same or no superscripts represent no significant differences at P > 0.05.

Journal: Poultry Science

Article Title: Dietary resveratrol supplementation alleviates cold exposure-induced pyroptosis and inflammation in broiler heart by modulating oxidative stress and endoplasmic reticulum stress

doi: 10.1016/j.psj.2024.104203

Figure Lengend Snippet: mRNA and protein expression levels of genes related to endoplasmic reticulum stress in broiler heart from each group. (A) Relative mRNA expression of GRP78, IRE1, PERK, EIF-2α, ATF4, ATF6, and CHOP in heart tissues. (B) Protein bands and expression levels of PERK, ATF4, and CHOP in heart tissues. a,b,c,d,e Bars with different superscripts in the same index are significantly different at P < 0.05, and bars with same or no superscripts represent no significant differences at P > 0.05.

Article Snippet: The membranes with proteins were blocked using a 5% of skim milk for 2 h, and then incubated with the corresponding primary antibodies against PERK (WL03378, 1:1000, Wanleibio, China), ATF4 (WL02330, 1:1000, Wanleibio, China), CHOP (WL00880, 1:1000, Wanleibio, China), NLRP3 (WL02635, 1:1500, Wanleibio, China), Caspase1 (WL02996, 1:1000, Wanleibio, China), GSDMD (AF4012, 1:1000, Affinity, China), IL-18 (WL01127, 1:1500, Wanleibio, China), IL-1β (WL00891, 1:500, Wanleibio, China), IL-10 (WL03088, 1:1000, Wanleibio, China), TNF-α (WL01581, 1:1500, Wanleibio, China), and β-actin (ab8227, 1:7000, Abcam, UK) for 12 h at 4°C.

Techniques: Expressing